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| 1. |
Put
the power of fully automated genetic
fingerprinting on your lab bench. |
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| When
you need to know more about the identity and relatedness of
bacterial samples , the RiboPrinter® Microbial
Characterization System is the tool for you . |
| It can provide a characteristic
genetic "fingerprint" or RiboPrint® pattern-a
characterization below the species level-for virtually any
bacterial isolate in about eight hours. Its built-in
database can also provide identification. And the RiboPrint®
system provides this information quickly and reliably , in a
compact , fully automated instrument that requires no
special skills to operate . |
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| 2. |
Spend
less time generating data and have more
time for other priorities. |
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| The RiboPrinter®
system's extraordinary throughput and 8-hour results let you
complete months of typing work in only days . |
| The RiboPrint®
system gives you the ultimate in flexibility combined with
speed and ease of use . The system's powerful built-in data
analysis software automatically clusters genetically similar
organisms. And the RiboPrint® system gives you
complete flexibility to choose restriction enzymes, without
sacrificing speed and automation. RiboPrint® patterns
are standardized, reliable and robust-a new language for
microbiology that gives you capabilities never before
possible . |
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| 3. |
| Use
a universal tool for the microbiology lab. |
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| The RiboPrinter®
system provides rapid, standardized identification and
characterization of the bacteria important to you. Results
are unambiguous and rapid, unlike the subjective results
obtained from biochemical-based tests or other manual typing
methods. |
The power
of the RiboPrint® system increases every time you
run a sample. The system automatically compares the RiboPrint®
pattern generated for each new sample to the patterns stored
for all other samples in your database. With a
comprehensive, dynamic picture of your microbial
environment, you now have the means to make microbiology
more efficient, saving time and money, in whatever area
you're working:
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QA/QC
and clinical trials |
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Epidemiology
and veterinary health investigations |
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R&D
and product development |
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Maintaining
the integrity of important cultures |
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| The RiboPrinter® system
is self-contained, reliable and easy to use. |
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| The RiboPrint®
system can automatically process up to eight bacterial
isolates at one time-with results available in about eight
hours from sample input. And since the system can accept new
batches every two hours, you can load up to 32 samples in a
normal working day. From start to finish, the process is
automated, simplifying operator training and minimizing
errors due to technique. |
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| Workstation |
| The
brains of the RiboPrint® system, it controls
the RiboPrint® system characterization unit,
analyzes and stores data and produces printed
reports, The workstation includes a personal
computer, keyboard, mouse, monitor, removable
hard disk drive, laser printer, modem, and
uninterruptible power supply. The proprietary
software provides user-friendly prompts, process
monitoring, diagnostics and complete data
analysis. |
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| Characterization unit |
| This
automated instrument performs DNA restriction
digestion, electrophoretic separation, membrane
transfer and detection-and automatically records
data on the accompanying workstation. Its
modular design simplifies troubleshooting and
maintenance. Loading and operation are easy and
intuitive, requiring little training. |
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| Membrane |
| Captures
the DNA fragments for final processing. |
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| Purified water |
| For
closed-system rehydration of dry disposables and
component rinsing. |
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| Sample carrier |
| Holds
eight samples. |
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| MP Base and inserts |
| Holds
the DNA probe, conjugate, and substrate (which
fuel the chemical reaction to create glowing
bands from the fragments of DNA). |
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| Gel Cassete |
| Holds
precast electrophoresis gel. Separates DNA
fragments into discrete bands. |
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| DNA prep pack |
| Restriction
enzyme and lysis buffer to break open bacteria,
release and cut DNA; marker DNA to help
normalize patterns. |
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| Sample preparation equipment |
| Sample
preparation is made rapid, safe and simple with
this specialized equipment. The hand-held mixer
disperses cells in the buffer solution. The Heat
Treatment Station inctivates bacterial samples
and renders them harmless. |
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The RiboPrint®
system process |
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| 1.Sample
preparation |
| This is the only step that
takes place outside the RiboPrint® characterization unit, Pure
colonies picked from a plate are inactivated by heat treatment. |
| 2.DNA
preparation |
| DNA is extracted from
bacterial cell lysate and cut into fragments by a restriction enzyme. |
| 3.Separation
and transfer |
| The DNA fragments are
separated according to molecular size by gel electrophoresis and are then
transferred to a membrane. |
| 4.Detection |
| After hybridization with a
labeled DNA probe, a chemiluminescent agent is introduced. The emission of
light from the hybridized fragments is then captured by a digitizing
camera and stored as image data. |
| 5.Data
processing |
| Using proprietary
algorithms, a RiboPrint® pattern for each sample is extracted from the
image data. This pattern is compared to other RiboPrint® patterns
stored in the system to characterize and identify the sample. |
| 6.Printed
report |
| A report that characterizes
and identifies the bacterium and includes its RiboPrint® pattern is
automatically printed for your review. Sophisticated data analysis tools
allow you to further process the information and share standardized data
among your network of users. |
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| The RiboPrinter® system
meets your needs |
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| Pinpoint
contamination. |
| The RiboPrint® system
can characterize and identify bacteria below the species
level so you can pinpoint the source contamination. |
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The RiboPrint®
pattern tells the story. Here, the finished
product contained Staphylococcus epidermidis.
but the Staphylococcus epidermidis found in the
raw material exhibits a different RiboPrint®
pattern.
The RiboPrint® pattern from a worker's hands
shows an exact match to the strain in the
finished product. The source of contamination is
rapidly and accurately pinpointed. |
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| Get
stable, standardized result. |
| RiboPrint® patterns
are reliable electronic data based on rRNA genes. They can
be analyzed, stored and shared with other users anywhere in
the world. |
| Hospital
1 |
| Hospital
2 |
| Hospital
3 |
| Hospital
4 |
| Hospital
5 |
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| RiboPrint®
pattern of Klebsiella pneumoniae isolates from
five hospitals show that hospitals 2 and 3 have
isolated the same strain. |
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| Characterize
virtually any bacterium. |
| The RiboPrint® system
lets you characterize environmental isolates, spoilage
organisms, pathogens-any bacterium of interest to you. |
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| Listeria
monocytogenes |
| Escherichia
coli O157: H7 |
| Bacillus
subtilus |
| Pseudomonas
aeruginosa |
| Enterococcus
faecium |
| Campylobacter
speicies cut with Pst 1 |
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