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The SURF-BLOT can be used for any technique that formerly required cutting the blot into strips . The SURF-BLOT eliminates this step by clamping liquid channels onto the surface of a blot . In a typical experiment , antigen is electrophoresed on a flat-topped gel ; the gel is transferred to blotting media and the resultant blot is clamped into the SURF-BLOT . Antibody solutions are then incubated in the liquid channels . After rinsing , the blot is removed from the SURF-BLOT and the blot visualization step is performed on the intact blot . Since there are no narrow strips to line up , the entire screening set can be compared on the intact blot . The SURF-BLOT 10 can also be used for "Grid-Blot " screening of monoclonal antibodies . this procedure also allows for rapid quantification of antibodies and antigens . The SURF-BLOT is the only blot screening device that has a convex stainless steel backing plate . Competitive designs rely upon the clamping of two pieces of flat plastic together , often leading to leakage between channels . |
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