For normalization of real-time PCR results in miRNA quantification in human, mouse, rat, and dog
For accurate and reproducible results in miRNA quantification by real-time PCR, it is necessary to normalize the amount of target miRNA by using a suitable endogenous reference RNA. This is known as relative quantification. miScript PCR Controls are primers that enable normalization of real-time PCR results in miRNA quantification studies using the miScript PCR System. miScript PCR Controls are available for a panel of 5 snoRNAs (SNORD61, SNORD68, SNORD72, SNORD95, and SNORD96A) and the snRNA RNU6B (RNU6-2). These controls are designed taking into consideration sequence homologies in human, mouse, rat, and dog so that the same control can be used for all 4 species. They can be ordered individually at the GeneGlobe Web portal.
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