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Brand / RAININ / 96爪微量分注系統

無縫克隆組裝技術的領導者
The world's most trusted method to build seamless high-fidelity DNA.


GIBSON ASSEMBLY® KITS 無縫克隆試劑

 高效率.高保真.無縫組裝多條DNA片段與載體克隆 Gibson Assembly® 較之傳統克隆技術不需要依賴限制性切位點進行核酸片段篩選,

而是藉由序列合成或是利用引子將延長核酸片段的末端,產生的同源交疊序列(Overlap), 通過這些同源交疊序列,

Gibson Assembly®可直接將不同的核酸片段無縫接合,組裝出長片段的線性或環狀dsDNA。Gibson Assembly®已被超過一千份文獻引用,

用於各種基因表達、基因編輯、基因體建構與免疫細胞治療等研究。

操作簡單,速度更快

支援多條核酸片段組裝,高克隆效率高達,

不需要任何限制性內切酶切位,兼容各種載體

Gibson Assembly®技術不需附加多餘序列,

實現高保真無縫克隆

Gibson Assembly® HiFi Kit

Gibson Assembly® Ultra Kit

Maximum # of fragments assembled: 1-5

Maximum # of fragments assembled: 1-15

Cloning efficiency: 90% full-length clones

Cloning efficiency: 95% full-length clones

Insert size range: 500–32,000 bp

Insert size range: 10–100,000 bp

Total construct size range:100,000 bp

Total construct size range:1,000,000 bp

Reaction time: 60 minutes

Reaction time:80 minutes

Protocol: One step

Protocol: Two steps

    Product Ordering information

    Product

    Bertec Cat. No.

    Gibson Assembly® HiFi Kit, (2x), 10 rxn, include Positive control

    CDXGA1100-10

    Gibson Assembly® HiFi Master Mix, (2x), 10 rxn

    CDXGA1100-10MM

    Gibson Assembly® HiFi Kit, (2x), 50 rxn, include Positive control

    CDXGA1100-50

    Gibson Assembly® HiFi Master Mix, (2x), 50 rxn

    CDXGA1100-50MM

    Gibson Assembly® HiFi Kit, (4x), 10 rxn, include Positive control

    CDXGA1100-4X10

    Gibson Assembly® HiFi Master Mix, (4x), 10 rxn

    CDXGA1100-4X10M

    Gibson Assembly® HiFi Kit, (4x), 50 rxn, include Positive control

    CDXGA1100-4X50

    Gibson Assembly® HiFi Master Mix, (4x), 50 rxn

    CDXGA1100-4X50M

    Gibson Assembly® Ultra Kit, (2x), 10 rxn, include Positive control

    CDXGA1200-10

    Gibson Assembly® Ultra Master Mix, (2x), 10 rxn

    CDXGA1200-10MM

    Gibson Assembly® Ultra Kit, (2x), 50 rxn, include Positive control

    Protocol: Two steps

    Gibson Assembly® Ultra Master Mix, (2x), 50 rxn

    Reaction time:80 minutes

      volume

      型號

      100 ul-1 ml

      RAN30296707

      5 ul-200 ul

      RAN30296706

      0.5 ul-20 ul

      RAN30296705

        *欲了解詳情或訂購請撥打伯昂免費電話:0800-362-362 (0800-DNA-DNA)

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            Tel. +886 2 2228 1234

            Fax +886 2 2228 1111

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