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Ni-NTA Spin Kit

For fast, small-scale purification of His-tagged proteins, includes spin columns, reagents, buffers, and control plasmid

  • Up to 300 μg His-tagged protein per column in as few as 15 minutes
  • Purification under native and denaturing conditions
  • Up to 95% homogeneity in one step
  • Ready-to-use spin columns for rapid automated or manual processing

Ni-NTA silica combines Ni-NTA with a macroporous silica support material optimized to suppress nonspecific hydrophobic interactions. The Ni-NTA Columns in the Spin Kit provide Ni-NTA silica in a convenient microspin format for easy preparation of multiple samples in parallel. They provide a simple method for functional screening of engineered proteins, selection of clones expressing full-length translation products, and comparison of expression levels. Each spin column can purify up to 300 µg of His-tagged protein. Like all Ni-NTA matrices, Ni-NTA spin columns can be used for one-step protein purification under native or denaturing conditions. The Ni-NTA Spin Kit is a complete kit for spin purification of His-tagged proteins.

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Nunc 96-Well UV Microplate
Cat. No. Surface Well Design Color Total Vol., μL Sterile Lid No. Per Pack No. Per Case
8404 UV 96 F Clear 392 No No 10 40

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