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PCR


miScript II RT Kit

  • For reverse transcription of total RNA containing miRNA using the miScript PCR System

     

    The miScript II RT Kit is an integral component of the miScript PCR System for miRNA detection and quantification. cDNA generated with the miScript II RT Kit is used as a template for real-time PCR with the miScript SYBR Green PCR Kit and either PCR arrays (miRNome or Pathway-Focused miScript miRNA PCR Arrays) or appropriate assays (miScript Primer Assays for miRNA or other noncoding RNA, miScript Precursor Assays for precursor miRNA, or QuantiTect Primer Assays for mRNA). 

     

    • - cDNA for sensitive and specific miRNA detection
    • - A single cDNA enables quantification of multiple miRNAs
    • - Quantification of miRNA and mRNA from the same cDNA
    • - Quantification of mature and precursor miRNA from the same cDNA
    • - Proprietary synthetic RNA to assess reverse transcription efficiency

miScript SYBR® Green PCR Kit

  • For miRNA detection by real-time PCR using the miScript PCR System

     

    The miScript SYBR Green PCR Kit is an integral component of the miScript PCR System for miRNA detection and quantification. The kit is based on the proven technology of QuantiTect SYBR Green Kits. cDNA generated by the miScript II RT Kit is used as the template for real-time PCR with the miScript SYBR Green PCR Kit and either PCR arrays (miRNome or Pathway-Focused miScript miRNA PCR Arrays) or appropriate assays (miScript Primer Assays for miRNA or other noncoding RNA, miScript Precursor Assays for precursor miRNA, or QuantiTect Primer Assays for mRNA).

     

    • - Sensitive and specific detection and quantification of miRNA
    • - Quantification of hundreds of miRNAs from a single cDNA
    • - Quantification of miRNA and mRNA from the same cDNA
    • - Includes ROX dye for optional normalization of fluorescence

QuantiNova probe PCR Kit

  • For highly sensitive, specific, and ultrafast, probe-based real-time PCR

     

    Avoid the deleterious effects of mispriming at lower temperatures with the QuantiNova Probe PCR Kit. Based on a novel, antibody-mediated, hot-start mechanism, the kit enhances the specificity and efficiency of probe-based real-time PCR to provide accurate, singleplex or duplex, cDNA or gDNA analysis on various real-time PCR cyclers. The added convenience of extreme stability for up to 100 hours at room temperature, without a need for any cooling agent, makes it ideal for handling of high-throughput samples and automated workflows. An in-built tracking system for visual identification of correct pipetting gives you absolute peace of mind while the extreme sensitivity for even low target amounts ensures reliable qPCR results every time.

     

    • - Accurate detection of rare targets down to one copy
    • - Duplex capability for more results per reaction
    • - Reaction stability of up to 100 hours at 30°C for flexible workflows
    • - Visual indication of correct pipetting resulting in fewer pipetting errors
    • - High specificity for better results
    • - Increased throughput due to ultrafast cycling

QuantiNova SYBR Green PCR Kit

  • For unparalleled results using SYBR Green-based Qpcr

     

    Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.

     

    • - Unmatched specificity due to novel hot-start mechanism
    • - Precise reaction setup with a built-in visual indicator
    • - Accurate and robust detection of targets down to one copy
    • - Magnified throughput and speed with ultrafast cycling
    • - Seamless performance with QuantiTect Primer Assays

RT2 SYBR® Green qPCR Mastermixes

  • For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays

     

    RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.

     

    • - Designed exclusively for use with RTand EpiTect Methyl II qPCR Assays
    • - Delivers specific amplification with minimal primer-dimers
    • - Available with ROX, fluorescein, or without dye
    • - For use with qPCR instruments under standard cycling conditions 

          QIAGEN Protease

          • For protease digestion during DNA and RNA preparation

          •  
          • QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions.

          •  
          • Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.

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