The PyroMark PCR Kit is specifically optimized for Pyrosequencing analysis, providing highly specific and unbiased amplification of template DNA for various applications, such as mutation detection, SNP analysis, methylation analysis, and sequencing. The kit is provided in a convenient master mix format consisting of HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer for highly specific amplification. In addition, Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC-rich) templates, is provided. The PyroMark PCR Kit is also supplied with CoralLoad Concentrate, which is strongly recommended for use with the PyroMark PCR Master Mix, for highly specific PCR and high yields of amplified DNA. CoralLoad Concentrate contains 2 gel-tracking dyes, allowing PCR products to be loaded directly on a gel and checked prior to Pyrosequencing.
The PyroMark Q24 uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q24 Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q24 Accessories and Reagents are designed specifically for use with the PyroMark Q24 instrument.
The QIAamp PowerFecal DNA Kit removes tough inhibitors from stool, gut and biosolid samples and provides clean, ready-to-use DNA for any downstream application. With the included Inhibitor Removal Technology, inhibitors from digested food, heme from lysed red blood cells in stool and other PCR inhibitors are eliminated leaving only high-quality DNA ready for downstream applications such as qPCR, Sanger sequencing and next-generation sequencing.
The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.
The QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. No mechanical homogenization is necessary as the tissues are lysed enzymatically. The convenient spin-column procedure reduces hands-on preparation time to 20 minutes. Purification of DNA using the QIAamp DNA Mini Kit can be automated on the QIAcube.
The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. Purification can be fully automated on the QIAcube.
The RNeasy Micro Kit is designed for purification of up to 45 μg RNA from small cell and tissue samples. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Micro Kit can be fully automated on the QIAcube Connect. For larger samples, the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.
The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 μg RNA. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube. For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.
Puregene Accessories used in combination with Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.
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