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Molecular biology


QIAGEN Proteinase K

  • For protease digestion during DNA and RNA preparation

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  • QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions.

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  • Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.

QIAzol Lysis Reagent

  • For efficient lysis of fatty and standard tissues before RNA isolation

     

    QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.

     

    • - Optimized lysis conditions to purify RNA for gene expression analysis
    • - High yields of RNA from fatty tissues
    • - Easy-to-follow protocol for lysis and homogenization
    • - Integration with RNeasy cleanup to prevent phenol carryover
    • - Compatibility with a variety of tissue types

RNAlater RNA Stabilization Reagent

  • For immediate stabilization of RNA in tissues

     

    RNAlater RNA Stabilization Reagent immediately stabilizes RNA in tissue samples to preserve the gene expression profile, and is supplied in 50 ml or 250 ml bottles. For stabilization of DNA, RNA, and protein in tissue samples, Allprotect Tissue Reagent can be used.

     

    • - Immediate RNA stabilization and protection
    • - Reliable gene expression and gene-profiling data
    • - Convenient and safe handling at room temperature
    • - No need for liquid nitrogen or dry ice
    • - Tissue archiving without risk of RNA degradation

RNAlater TissueProtect Tubes

  • For collection, RNA stabilization, transport, and storage of tissues

     

    RNAlater TissueProtect Tubes are screw-top tubes that contain RNAlater RNA Stabilization Reagent, which immediately stabilizes RNA in tissue samples to preserve the gene expression profile. Stabilization takes place a room temperature, eliminating the need for dry ice or liquid nitrogen.

     

    • - Immediate RNA stabilization and protection
    • - Reliable gene expression and gene-profiling data
    • - Convenient and safe handling at room temperature
    • - Tissue archiving without risk of RNA degradation
    • - Easy collection and storage in reclosable tubes

RNase A

  • For RNase digestion during DNA preparation

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  • QIAGEN Ribonuclease A (RNase A) is endonuclease-free and quality-controlled for use in plasmid purification procedures for digestion of RNA.

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  • This ready-to-use solution has the same specifications as the RNase supplied in all QIAGEN plasmid DNA purification kits.

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  • An aliquot of the solution must be diluted into Buffer P1 to the appropriate concentration.

          RNase-Free DNase Set

          • For DNase digestion during RNA purification

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          • The QIAGEN RNase-Free DNase Set is guaranteed RNase-free, quality-controlled, and optimized for use with RNeasy procedures and with QIAamp RNA Blood Mini procedures. Generally, DNase digestion is not required for RNA purified with RNeasy Kits since the silica-gel–membrane, spin-column technology efficiently removes the majority of the DNA without DNase treatment.

          • However, more complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA. Buffer RDD, included in the set, is optimized for on-column DNase digestion of 15 minutes at 20–30°C.

          • The buffer is also well-suited for efficient DNase digestion in solution. The RNase-Free DNase Set provides efficient on-column digestion of DNA during RNA purification from cells and tissues using RNeasy Kits and the QIAamp RNA Blood Mini Kit. The DNase is efficiently removed in subsequent wash steps.

          • For DNase treatment when using the RNeasy 96 Kit, please contact QIAGEN Technical Services or your local distributor for a separate, optimized protocol.

          • The QIAGEN RNase-Free DNase Set is delivered as a stable, lyophilized enzyme. The RNase-Free DNase Set provides 1500 Kunitz units. One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per milliliter at 25°C, pH 5.0, with highly polymerized DNA as the substrate.

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