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- 有效且完整的裂解組織細胞並穩定蛋白質
- 可獲得胞內、細胞膜與細胞核內蛋白質
- 不使用SDS(石油來源)
- 使用有機介面活性劑
- 可相容多種蛋白酶抑制劑
型號: ECORIPA-100
- Efficient and complete cell lysis and solubilization of proteins.
- Cytoplasmic, membrane and nuclear proteins.
- No SDS.
- Organic surfactant.
- Compatible with a wide range of individual protease inhibitors and cocktails.
- 有效且完整的裂解組織細胞並穩定蛋白質
- 可獲得胞內、細胞膜與細胞核內蛋白質
- 不使用SDS(石油來源)
- 使用有機介面活性劑
- 可相容多種蛋白酶抑制劑
型號: ECORIPA10x
- Convenience: It combines RIPA Lysis Buffer and Laemmli Sample Buffer in 1 solution.
- Denser bands: It allows for thicker bands especially when working with small number of cells, lysates with low concentration, and proteins with low expression.
- Ease of use: ClearBand Lyse&Load is easily loaded into the wells thanks to its unique content.
- Quick: No loss of time with freeze and thaw, since it is stored at room temperature.
- 方便:結合RIPA裂解液與蛋白質上樣緩衝液
- 條帶加深:細胞數小、低濃度裂解物、低濃度表現蛋白的檢體可以使條帶變深
- 容易使用:很容易可以把細胞裂解物加樣至電泳膠中(不需另行添加蛋白質上樣緩衝液)
- 快速:不浪費時間重複解凍、儲存於室溫
型號: ECOLL5ML
ClearBand Cytoplasmic & Nuclear Protein Fractionation Kit 細胞質/核蛋白質萃取試劑
- For stepwise separation and preparation of cytoplasmic and nuclear extracts from mammalian cultured cells or tissue.
-The extracted proteins are suitable for SDS-PAGE, Western blot, ELISA, immunoprecipitation, transcription factor- and enzyme activity assays.
- It generally have less than 10% contamination between nuclear and cytoplasmic fractions.
- 有效分離萃取哺乳類細胞與組織檢體的細胞質與細胞核蛋白質
- 萃取所得的蛋白質適用於SDS-PAGE/ 西方墨點法/ ELISA/ 免疫沉澱/ 轉錄因子酵素活性等後續實驗之用
- 細胞核與細胞質間, 蛋白質的交互污染低於10%
型號: ECOCNF100
ClearBand 100x Protease Inhibitor Cocktail 磷酸酶抑制劑
- Contains six broad-spectrum protease inhibitors (AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, and Pepstatin A) in DMSO, that powerfully inhibits serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases appearing in cellular lysate samples.
- Suitable for use in following applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
- 含有六種廣譜蛋白酶抑制劑(AEBSF、Aprotinin、Bestatin、E-64、Leupeptin 和 Pepstatin A),能有效抑制細胞裂解樣本中的絲氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶及氨基肽酶
- 適用於以下應用:西方墨點法、免疫共沉澱、免疫沉澱、免疫螢光、免疫組織化學染色、流式細胞、酵素活性測定等
型號: ECOPIC1, ECOPIC5
ClearBand Bradford Reagent 蛋白質定量試劑組
- Ready-to-use.
- Quick measurement of total protein concentration.
- Contains Coomassie Brilliant Blue G-250, which associates with basic and aromatic amino acids, thus leading to a shift in absorbance during protein determination.
- 即用型溶液
- 快速測量總蛋白質濃度
- 含有考馬斯亮藍 G-250,它與鹼性和芳香族氨基酸結合,從而導致蛋白質測定過程中吸光度發生變化
型號:ECOBR05
ClearBand Ponceau S 麗春紅蛋白質染劑
- Ready-to-use.
- Rapid (5 min) and reversible detection of protein bands on nitrocellulose, PVDF, cellulose acetate and membranes.
- 即用型溶液
- 快速(5 分鐘)可逆性的檢測硝酸纖維素膜、PVDF膜、醋酸纖維素膜上的蛋白質條帶
型號:ECOPS05, ECOPS052
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