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Purification


QIAcube and QIAcube Connect Accessories

  • For automated purification of DNA, RNA, or proteins on the QIAcube and QIAcube Connect

  •  
  • QIAcube and QIAcube Connect accessories are for use with the QIAcube and QIAcube Connect instruments for fully automated sample preparation using QIAGEN spin-column kits.

QIAamp Circulating Nucleic Acid Kit

  • For isolation of free-circulating DNA and RNA from human plasma or serum

     

    The QIAamp Circulating Nucleic Acid Kit greatly simplifies concentration and purification of free-circulating DNA and RNA from plasma or serum.

     

    • - Concentration of nucleic acids, with high input and low elution volumes
    • - Efficient recovery of fragmented DNA and RNA
    • - No organic extraction or ethanol precipitation
    • - Removal of contaminants and inhibitors
    •  

    The kit can be automated on the QIAcube Connect.

QIAamp PowerFecal DNA Kit

  • For the isolation of DNA from stool, gut material and biosolids

     

    The QIAamp PowerFecal DNA Kit removes tough inhibitors from stool, gut and biosolid samples and provides clean, ready-to-use DNA for any downstream application. With the included Inhibitor Removal Technology, inhibitors from digested food, heme from lysed red blood cells in stool and other PCR inhibitors are eliminated leaving only high-quality DNA ready for downstream applications such as qPCR, Sanger sequencing and next-generation sequencing.
     

    • - High-quality DNA from up to 250 mg samples in just 30 minutes
    • - Optimized for tough DNA samples from stool, biosolids and gut material
    • - Efficient removal of downstream inhibitors with Inhibitor Removal Technology

QIAamp DNA Micro Kit

  • For purification of genomic and mitochondrial DNA from small samples

     

    The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.

     

    • - Rapid purification of high-quality DNA
    • - No organic extraction or alcohol precipitation
    • - Consistent, high yields
    • - Complete removal of contaminants and inhibitors

QIAamp DNA Mini Kit

  • For isolation of genomic, mitochondrial, bacterial, parasite, or viral DNA

     

    The QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. No mechanical homogenization is necessary as the tissues are lysed enzymatically. The convenient spin-column procedure reduces hands-on preparation time to 20 minutes. Purification of DNA using the QIAamp DNA Mini Kit can be automated on the QIAcube.

     

    • - Rapid purification of high-quality, ready-to-use DNA
    • - Consistent, high yields
    • - Complete removal of contaminants and inhibitors

          QIAamp RNA Blood Mini Kit

          • For purification of cellular RNA from fresh whole blood

             

            The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. Purification can be fully automated on the QIAcube.

             

            • - Rapid purification of high-quality, ready-to-use RNA
            • - No organic extraction or alcohol precipitation
            • - Consistent, high yields
            • - Removal of contaminants and inhibitors

          RNeasy Micro Kit

          For purification of up to 45 µg total RNA from cell and tissue samples

           

          The RNeasy Micro Kit is designed for purification of up to 45 μg RNA from small cell and tissue samples. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Micro Kit can be fully automated on the QIAcube Connect. For larger samples, the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

           

          • - Fast procedure delivering high-quality total RNA in minutes
          • - Ready-to-use RNA for high performance in any downstream application
          • - Consistent RNA yields from very small amounts of starting material
          • - No phenol/chloroform extraction
          • - No CsCl gradients, no LiCl or ethanol precipitation

          RNeasy Mini Kit

          For purification of up to 100 µg total RNA from cells, tissues, and yeast

           

          The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 μg RNA. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube. For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

           

          • - Fast procedure delivering high-quality total RNA in minutes
          • - Ready-to-use RNA for high performance in any downstream application
          • - Consistent RNA yields from small amounts of starting material
          • - No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation

            Puregene Accessories

            For purification of archive-quality DNA from a wide range of sample types

             

            Puregene Accessories used in combination with Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.

             

            • - Archive-quality DNA for long-term storage
            • - Reproducible DNA purification
            • - Convenient, scalable purification procedure

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